Phylogenetic analysis of Pectinidae(Bivalvia) based on the ribosomal DNA internal transcribed spacer region

The ribosomal DNA internal transcribed spacer (ITS) region is a useful genomic region for understanding evolutionary and genetic relationships. In the current study, the molecular phylogenetic analysis of Pectinidae (Mollusca: Bivalvia) was performed using the nucleotide sequences of the nuclear ITS region in nine species of this family. The sequences were obtained from the scallop species Argopecten irradians, Mizuhopecten yessoensis, Amusium pleuronectes and Mimachlamys nobilis, and compared with the published sequences of Aequipecten opercularis, Chlamys farreri, C. distorta, M. varia, Pecten maximus, and an outgroup species Perna viridis. The molecular phylogenetic tree was constructed by the neighbor-joining and maximum parsimony methods. Phylogenetic analysis based on ITS1, ITS2, or their combination always yielded trees of similar topology. The results support the morphological classifications of bivalve and are nearly consistent with classification of two subfamilies (Chlamydinae and Pectininae) formulated by Waller. However, A. irradians, together with A. opercularis made up of genera Amusium, evidences that they may belong to the subfamily Pectinidae. The data are incompatible with the conclusion of Waller who placed them in Chlamydinae by morphological characteristics. These results provide new insights into the evolutionary relationships among scallop species and contribute to the improvement of existing classification systems.     

珠江口近30a的SST变化特征分析

根据珠江口多个定点站近30a表层海水温度（SST）实测资料和气象站观测的气温资料，使用功率谱和小波分析方法，计算分析珠江口SST变化特点、上升趋势及其与El Nino／La Nino的响应关系．结果表明各月SST存在着1-2℃的海域差异，SST的年较差达10～11℃，SST的季节变化与全球气候变暖呈显著相关．1971-2003年SST呈显著上升趋势，其线性上升率为0．019～0．034℃／a，且珠江口外高于口内．El Nino／La Nino事件对珠江口SST变化的影响并非单一的对应关系．     

柘林湾表层沉积物重金属分布及污染初步评价

研究了柘林湾表层沉积物样品中7种重金属元素的质量分数及分布特征,并对其来源进行了分析。结果显示,调查海区Zn的平均质量分数为113.0μg/g±6.5μg/g、Cr为40.1μg/g±8.5μg/g、Mn为837.9μg/g±168.6μg/g、Cu为24.0μg/g±4.3μg/g、Pb为57.7μg/g±11.3μg/g、Ni为24.4μg/g±5.7μg/g、Fe为3.7%±0.4%。重金属主要来源于陆源风化产物,其质量分数反映了粤东地区的地质特点。分别以沉积物质量和香港环保署公布的标准,对柘林湾表层沉积物重金属污染进行了评估,结果表明:只有Pb在部分站位达到中度污染水平,其它重金属元素则只为轻度污染。     

粤东柘林湾表层沉积物中的多环芳烃

近岸海域是绝大数污染物的最终归宿,其中多环芳烃(PAHs)的污染目前已成为国内外环境问题的焦点之一[1~3].20世纪80年代以来,国内外对河口湾、港湾、近岸海域等海洋环境中PAHs的含量、组成、分布、归趋及来源等做了大量的调查[4~15].比如,中国科学院广州地化所对珠三角水域和厦门大学对厦门港一带均做了大量的工作,结果发现表层沉积物中16种优控PAHs总量在珠江口达到156~9220 ng/g,闽江口为175~817ng/g,厦门西港则在196~675 ng/g之间,证明我国东南沿海主要河口及港湾沉积物中的PAHs污染已经达到中等水平[13~15].     

氨氮胁迫对中华绒螯蟹(Eriocheir sinensis) 免疫功能的影响

在实验室条件下构建不同氨氮浓度环境和养殖时间的组间差异,研究氨氮胁迫对中华绒螯蟹免疫功能的影响。结果表明,中华绒螯蟹在NH 4-N3.0mg/L10天或1.0mg/L20天时,其血淋巴血细胞密度极显著地低于对照组(P<0.01)。NH4 -N2.0mg/L和3.0mg/L无论10天或20天,血细胞吞噬百分率均分别为显著(P<0.05)和极显著(P<0.01)地低于对照组。NH4 -N3.0mg/L10天或2.0mg/L20天,血细胞吞噬指数显著低于对照组(P<0.05)。NH4 -N1.0mg/L和2.0mg/L分别20天时,血清溶菌酶(LSZ)活力分别为显著(P<0.05)和极显著(P<0.01)地低于对照组。NH4 -N1.0—5.0mg/L10天时,血清酚氧化酶(PO)活力均极显著(P<0.01)下降;但NH4 -N1.0—4.0mg/L20天时,血清PO活力水平均比10天时有所上升。NH4 -N3.0—4.0mg/L10天或1.0—2.0mg/L20天,以及5.0mg/L10天或3.0—5.0mg/L20天时,超氧化物歧化酶(SOD)活力分别为显著(P<0.05)和极显著(P<0.01)地低于对照组。上述结果表明,随着氨氮胁迫浓度的增加和胁迫时间的延长,会引起中华绒螯蟹血细胞数量、血细胞吞噬能力、溶菌酶活力、酚氧化酶活力和超氧化物歧化酶活力等的逐渐下降,使机体非特异性免疫防御系统遭到损伤,同时机体清除自由基的能力下降,机体细胞和组织受到伤害甚至出现死亡。     

96孔酶标板法测定中国对虾血淋巴上清液抗菌活力和酚氧化酶活性的初步研究

用96孔酶标板法测量单尾中国对虾血淋巴上清液抗菌（Uα）应力和过氧化酶活性。实验表明，该法具有以下优点：1）可测定大批单尾对虾血淋巴上清液的抗菌活力，酚氧化酶活性；2）所需对虾血淋巴上清液微量化；3）用酶标仪读取酶的活性，减少了不同试验者人为读数时的误差；4）重复性高。     

光诱鱿鱼浮拖网渔具渔法试验报告

1989－1999年开展光诱鱿鱼浮拖网渔具渔法研究的生产试验结果为：1998年生产试验86个夜（航次），总产量33．225t，其中鱿鱼产量为12．470t，占总产量的37．5％，总产值139000元，比同村光诱敷网的平均产量（31．62t）高5．1％左右。1999年生产试验80夜（航次），总产量54．66t，其中鱿鱼产量31．92t，占总产量的58．4％，总产值187000元。试验网产量比同村光诱敷网的平均产量（45．64t)增产19．76％，产值增加22．65％，鱿鱼产量占总渔获量的比例提高近10个百分点，投入产出比为1：1．684。取得了较好的试验结果，达到了预期试验目的。此外，文中还讨论了水上灯数量，网具规格和柔性网板等对生产效果和捕捞对象的影响及适应性情况，并建议开展光场强度及其分布的海上实测和光场强度对鱿鱼，趋光性鱼类的行为习性以及生长影响等试验研究，制定出光诱鱿鱼浮报网和敷网作业诱鱼灯数量和分布标准，以合理利用鱿鱼和中上层鱼类资源，实现可持续发展。     

西江河口重金属迁移扩散数值模拟的研究

本文以水体中重金属(包括溶解态和颗粒态)的物理迁移过程为基础,建立重金属迁移扩散的数学模式。模式中考虑悬浮物沉降和再悬浮对水体中重金属浓度的影响,并考虑咸潮活动引起悬浮物的絮凝沉淀作用。计算结果表明,这种潮汐河口区重金属迁移扩散模式基本上是成功的。并以镉为例,说明重金属在西江河口区迁移扩散的特点。     

MARINE BIOLOGICAL CHORUSES OBSERVED IN XIAMEN HARBOUR

Marine biological choruses observed in Xiamen harbour from August 1981 to October 1982 lasted for a few hours each time during the neap tide in spring, summer and autumn, in both the evening and the morning, with molt energy at frequencies between 700 Hz and 1.6 kHz and spectral peaks mainly at 800 Hz, 1 kHz and 1.25 kHz. During choruses, noise spectrum levels rose up to 46 dB higher than the background noise in the sea Mate 0. The statistics of occurrence of the evening choruses indicate that they might be fairly widespread in and near shallow waters of the region. Sources of such choruses are likely to be attributed to Johnnius belengerii and Collichthys lucidut of the family Sciaenidae.     

AN ECOLOGICAL STUDY OF FOULING ORGANISMS IN XISHA ISLANDS

Monthly and seasonal panel experiments were carried out in the Yongxing Island from September 1979 to August 1981. Altogether 119 species of fouling organisms are collected, of which, Hydroides elegans, Serpula vermicular is, Scrupocellaria longispinosa, Amathia distant, Enteromorpha spp. and Ostrea spp. are dominant species. Fouling of organisms may occur all the year round with evident seasonal variations, Species succession in the process of community formation is very evident and the climax has arrived after two years. Great variation exists between fouling organisms in different years and at different stations.